Bioactivities of a tumour necrosis-like factor released by chicken macrophages

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Bioactivities of a tumour necrosis-like factor released by chicken macrophages.

To test for tumour necrosis-like factor (TNF) of chickens, supernatants of a lipopolysaccharide (LPS)-stimulated chicken macrophage cell line MQ-NCSU were analysed. A sequence of ion-exchange and gel-permeation chromatography was utilised to isolate TNF-like activity from the culture supernatant. The peak of TNF-like cytotoxic activity corresponded to the fractions with a molecular weight of 81...

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ژورنال

عنوان ژورنال: Developmental & Comparative Immunology

سال: 1999

ISSN: 0145-305X

DOI: 10.1016/s0145-305x(99)00043-9